H-DBAS - Human-transcriptome DataBase for Alternative Splicing

Materials and Methods

Resources

Human full-length cDNA - Sequnece providers
Human mRNA - http://h-invitational.jp/hinv/download/README_62.txt
Mouse full-length cDNA - Sequnece providers
Mouse mRNA: H-InvDB 6.2 (DDBJ 73)
Chimpanzee RNA: H-InvDB 5.3 (DDBJ 69) + RefSeq 23 + Ensembl 44
Macaque RNA: H-InvDB 5.3 (DDBJ 69) + RefSeq 23 + Ensembl 44
Rat RNA: H-InvDB 5.3 (DDBJ 69) + RefSeq 23 + Ensembl 44
Dog RNA: H-InvDB 5.3 (DDBJ 69) + RefSeq 23 + Ensembl 44

Mapping and clustering pipelines (Information from H-InvDB)

Scheme - http://h-invitational.jp/hinv/help/contents2/clusters.html

ORF prediction (Information from H-InvDB)

Flowchart - http://h-invitational.jp/hinv/help/contents2/proteins.html

Truncated transcript decision

Scheme

Representative AS variant (RASV) decision

Genomic comparison analysis of RASVs between human and other species

Scheme