Experimental confirmation for the separation of sub-cellular fractions

Table 1 PCR primers of sca/snoRNAs (nuclear RNAs) for real time RT-PCR analysis

targetfold (N/C)PCR primerPCR primer
scaRNA249.4ACGCGTGAGTGTGTGAGTGTGCAGGAGGAGAGCTTTTCATT
scaRNA1276.5TGATGAGACTAAGGCGAATGCGCACCAGAAATGAAGGCAAG
scaRNA1047.2AATCTTGGTGGGCGATACAGCCCTGATACCCTGAACATGC
scaRNA1325.0GTAGTCTTGGAGCCGCACAGGTGGCAACAGTGACCAGAAA
snoRNA73103.5CTCTGTCCAAGTGGCGTAGGGACAGGACTCTGGGAAGCTG




Figure 1 Separated sub-cellular fractions (cytoplasm, nuclear and polysome) by real time RT-PCR of sca/snoRNAs (nuclear RNAs) and Western blotting of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (cytoplasmic protein) and Lamin A/C (nuclear protein).